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. 2011 Dec 16;287(7):4972–4981. doi: 10.1074/jbc.M111.297218

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — RGS6 complex formation in cerebellum and cerebrum. A, RGS6 co-immunoprecipitates with Gβ₅ and R7BP but not GIRK1 and GIRK2 in cerebellar and cerebral lysates. Actin serves as a loading control for Western blots. Input represents total tissue lysates used for subsequent immunoprecipitation. B, immunoprecipitation of GIRK1 from hippocampus, cerebrum, and cerebellum fails to reveal complex formation between GIRK1 and RGS7, Gβ₅, or RGS6. Input represents blots from isolated membrane fractions used for subsequent immunoprecipitation and Gα_i3 served as a loading control for Western blots. NS = nonspecific immunoreactive band, IgG = immunoglobulin heavy chain.