FIGURE 4.

Sedimentation velocity analyses of dimeric and trimeric MBL. The c(s) size distribution analyses correspond to the sedimentation velocity experiments performed at a rotor speed of 50,000 rpm. The concentrations of dimeric and trimeric MBL were 0.37 and 0.15 mg/ml, respectively. A and C, black outlines represent the experimental interference data, and the continuous white lines represent the boundary fits. For the dimer (A) and trimer (C), every 10th boundary of up to 300 scans was fitted, and only every 20th boundary is shown for clarity. B and D, c(s) analyses for the MBL dimer (B) and MBL trimer (D). The peaks corresponding to the monomer (M), dimer (D), trimer (Tri), and tetramer (Tet) are labeled.