FIGURE 1.

RA-FLS express the KCa1.1 channel α subunit. A, representative RT-PCR on RNA extracted from HEK 293 cells stably transfected with the KCa1.1 channel α subunit, non-transfected HEK 293 cells, and RA-FLS. B, representative Western blot against the KCa1.1 α subunit on total protein extracts from HEK 293 cells stably transfected with KCa1.1 channels, non-transfected HEK 293 cells, and RA-FLS. C, immunocytochemistry staining of RA-FLS for confocal microscopy. Left, wheat germ agglutinin (WGA) with membrane staining. Middle left: anti-KCa1.1 α subunit antibody. Middle right, DAPI showing nuclear staining. Right, merged images.