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CA mTOR increases PTEN to regulate phosphorylation of Akt in 293 cells. 293 cells were transfected with CA mTOR or vector plasmids. Twenty-four hours post-transfection, the cells were incubated in serum-free medium for 24 h prior to harvest. A, total RNA was analyzed for expression of PTEN and GAPDH mRNAs by semiquantitative RT-PCR. B, cleared cell lysates were immunoblotted with the indicated antibodies. p-Akt, phospho-Akt; S6K, S6 kinase; p-S6K, phospho-S6 kinase.
