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. 2011 Dec 19;287(6):3808–3822. doi: 10.1074/jbc.M111.246397

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Down-regulation of rictor or mSin1 decreases phosphorylation of 4EBP-1. 293 cells were transfected with two independent shRNA plasmids targeting either rictor (A and B) or mSin1 (C and D) or with scrambled (Scr) vector as indicated. The cell lysates were immunoblotted with phospho-4EBP-1 (p-4EBP-1) (Thr-37/46), phospho-4EBP-1 (Ser-65), and 4EBP-1 antibodies. Immunoblotting of the same samples with antibodies against rictor, mSin1, phospho-Akt (p-Akt) (Ser-473), Akt, and actin is also shown.