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. 2011 Dec 19;287(6):3808–3822. doi: 10.1074/jbc.M111.246397

FIGURE 9.

FIGURE 9.

TORC2 regulates Hif1α-driven PTEN expression. A–D, 293 cells were transfected with HA-tagged Hif1α along with either shRictor 1 (A), shRictor 2 (B), mSin1 sh1 (C), or mSin1 sh2 (D). Forty-eighty hours post-transfection, the cells were lysed, and the lysates were immunoblotted with the indicated antibodies. E and F, TORC2 regulates Hif1α-dependent PTEN transcription. 293 cells were cotransfected with PTEN-Luc and HA-Hif1α along with shRictor 1 (E), shRictor 2 (F), mSin1 sh1 (G), or mSin1 sh2 (H). Forty-eight hours post-transfection, luciferase activity was determined in the cell lysates. Mean ± S.E. of six determinations is shown. In E, * represents p < 0.01 versus control, # represents p < 0.001 versus control, and ** represents p < 0.001 versus shRictor 1 alone. In F, * represents p < 0.001 versus control, # represents p < 0.001 versus control, and ** represents p < 0.001 versus shRictor 2 alone. In G, * represents p < 0.001 versus control, # represents p < 0.001 versus control, and ** represents p < 0.001 versus mSin1 sh1 alone. In H, * represents p < 0.01 versus control, # represents p < 0.001 versus control, and ** represents p < 0.001 versus mSin1 sh2 alone. Scr, scrambled; p-Akt, phospho-Akt.