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. 2011 Dec 19;287(6):3823–3832. doi: 10.1074/jbc.M111.323337

FIGURE 3.

FIGURE 3.

APC directly enhances GSK-3 activity. In vitro kinase reactions were carried out using purified recombinant proteins. A, addition of the SAMP fragment (80 nm) of APC, but not BSA, directly enhances Tau phosphorylation (Ser-396/404) by GSK-3 by ∼5 fold. For image quantification, phospho-Tau was normalized to total Tau and then all lanes were normalized to lane 2. B, SAMP, but not BSA, directly enhances β-catenin phosphorylation (Ser-33/37/41) by GSK-3 by ∼7-fold. For image quantification, phospho-β-catenin was normalized to total β-catenin and then all lanes were normalized to lane 3. C, SAMP enhances β-catenin phosphorylation by GSK-3 in the presence of Axin and CKI by ∼2-fold. For image quantification, phospho-β-catenin was normalized to total β-catenin and then lane 2 was normalized to lane 1.