FIGURE 4.

Wnt signaling disrupts the interaction between APC and Axin. L cells were treated with Control or Wnt3a conditioned medium (CM) for indicated times. A, APC was immunoprecipitated from control and Wnt3a-treated cells, and samples were immunoblotted for Axin or APC. For image quantification, Axin was normalized to APC and then all lanes were normalized to control (lane 1). Error bars represent S.E. of four independent experiments. B, lysates from panel A were immunoblotted for β-catenin, Axin, APC, and GAPDH. β-catenin accumulation in response to Wnt activation correlates with Axin/APC dissociation in panel A. C, Wnt3a does not affect GSK-3 association with Axin.