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. 2011 Dec 19;287(6):3823–3832. doi: 10.1074/jbc.M111.323337

FIGURE 5.

FIGURE 5.

LRP6 binding to Axin causes APC dissociation. A, a constitutively active form of LRP6 (VSV-G-CA-LRP6) was expressed in 293T cells with Axin, and cell lysates were immunoprecipitated with antibodies to VSV-G, Axin, or control antibody, and then immunoblotted for APC, Axin, or VSV-G-CA-LRP6. Axin IP samples were adjusted for equal loading of Axin. Constitutively active LRP6 reduces APC association with Axin (compare lanes 3 and 4). LiCl (15 mm) reduces LRP6/Axin association and restores APC/Axin association (lane 5). APC association with CA-LRP6 was not detected (lane 2). B, Western blot analysis of APC, Axin, and CA-LRP6 in lysates used for immunoprecipitation in panel A. Co-expression of Axin and CA-LRP6, but not LRP6ΔN1ab, increased Axin levels for unclear reasons. C, unphosphorylatable VSV-G-LRP6ΔN1ab does not associate with Axin and does not affect Axin/APC interaction. D, 15 mm LiCl alone does not affect Axin/APC interaction.