FIGURE 3.

Inhibition of Dab-1, PI3K, PKCζ, and Sp1 reduces ABCA1 mRNA expression induced by reelin and apoE3. A and B, mouse macrophages were treated with 50 μm LY294002, 8 μm 109203X, 100 nm mithramycin A (mmA) or culture medium alone for 30 min and then treated with 3 μg/ml apoE3, 2 μg/ml reelin, or culture medium (Control) for 1 h. C, macrophages were transfected with a kinase-dead PKCζ mutant or an empty pEGFP vector. D, macrophages were transfected with Dab1 siRNA or control siRNA. The transfected cells were treated with 2 μg/ml reelin, 3 μg/ml apoE3, or culture medium alone (Control) for 1 h. ABCA1 mRNA levels were determined by quantitative real-time RT-PCR and normalized to β-actin mRNA. Values represent the mean ± S.E. of three separate experiments. *, p < 0.05 compared with cells without reelin or apoE3 treatment; †, p < 0.05 compared with cells treated with reelin or apoE3 alone; #, p < 0.05 compared with cells transfected with empty vector or control siRNA alone; $, p < 0.05 compared with cells treated with apoE3/reelin and transfected with empty vector or control siRNA.