FIGURE 4.

Knockdown of VLDLR and apoER2 reduces apoE3- and reelin-induced ABCA1 mRNA expression. Mouse macrophages were transfected with siRNA against VLDLR or apoER2 or control (Ctrl) siRNA. A and B, protein levels of apoER2 and VLDLR were determined by Western blot analysis and normalized to β-actin. C, transfected cells were incubated with 2 μg/ml reelin, 3 μg/ml apoE3, or culture medium alone for 1 h. ABCA1 mRNA levels were determined by quantitative real-time RT-PCR and normalized to β-actin mRNA. Values represent the mean ± S.E. of four independent experiments. *, p < 0.05 compared with cells transfected with control siRNA; †, p < 0.05 compared with cells transfected with same siRNA and untreated with apoE3 or reelin (control), and #, p < 0.05 compared with cells transfected with control siRNA and treated with apoE3 or reelin.