TABLE 1.

Reelin and apoE3 increase apoAI-mediated cholesterol efflux

Mouse macrophages were preincubated for 24 h with [³H]cholesterol in the presence or absence of mouse apoB-carrying lipoproteins. After washing with PBS, 3 μg/ml apoE3 and 2 μg/ml reelin or culture medium alone (control) were added and incubated for an additional 2 h. The radioactivity (dpm) in the medium and the cell lysates was then measured.

Treatments	Medium	Cell lysate	Cholesterol effluxa	ApoAI-mediated effluxb
	dpm	dpm	%	%
Without apoAI
Control	104 ± 9	38362 ± 4072	0.27 ± 0.04
ApoE3	138 ± 6	39427 ± 3610	0.35 ± 0.05
Reelin	114 ± 16	39310 ± 4258	0.29 ± 0.04
With apoAI
Control	261 ± 17	39574 ± 5038	0.66 ± 0.06	0.39 ± 0.05
ApoE3	937 ± 71c	39886 ± 3077	2.35 ± 0.29c	2.00 ± 0.24c
Reelin	640 ± 103c	37854 ± 4908	1.69 ± 0.22c	1.40 ± 0.21c

^a Cholesterol efflux was expressed as the percentage of radioactivity in the medium compared to the total radioactivity (cell lysate plus medium).

^b ApoAI-mediated cholesterol efflux was calculated as the difference between the values obtained in the presence or absence of ApoAI in the medium. Values represent the mean ± S.E. of four independent experiments.

^c p < 0.05 compared to controls.