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Comparison of kinetic parameters for the reaction of SsOGT and SsOGT-H5 mutant
Constants are obtained by fitting the reciprocal of data to the linearized Equation 1 shown under “Experimental Procedures.”
| ds-UPm | k | KVG | |
|---|---|---|---|
| μm | s−1 | μm | |
| SsOGT | 0 | 0.12 ± 0.01 | 4.66 ± 0.07 |
| 1.25 | 0.13 ± 0.01 | 23.16 ± 0.01 | |
| SsOGT-H5 | 0 | 1.1 ± 0.4 | 36.44 ± 7.96 |
| 1.25 | 1.2 ± 0.3 | 37.54 ± 5.85 |
