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. 2011 Dec 8;287(6):4023–4032. doi: 10.1074/jbc.M111.314450

FIGURE 1.

FIGURE 1.

Expression of a R-protein NBD. A, protein domains of Os02g_25900. CC, coiled coil domain; NBD, nucleotide binding domain; ARC, Apaf-1, R-protein, CED-4 domain, LRR, leucine-rich repeat. B, analysis of protein co-purifying with R1-NB (SDS/PAGE analysis and Coomassie Blue staining). 10 μl aliquots of protein from successive 1 ml of anion exchange column fractions were assayed for nucleotidase activity using ADP as substrate and applied to an SDS-PAGE gel. Molecular mass standards (in kDa) are indicated. Eluted proteins were identified by trypsin digest and MALDI-TOF. C, purification of recombinant R1-NB (SDS/PAGE analysis and Coomassie Blue staining). A 1.5 μg portion of protein was applied and molecular mass standards (in kDa) are indicated.