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. 2011 Dec 8;287(6):4023–4032. doi: 10.1074/jbc.M111.314450

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Purification and assay of recombinant NB-ARC domains (SDS/PAGE analysis and Coomassie Blue staining). A, purification of recombinant PSiP-NBARC. A 1.5 μg portion of protein was applied and molecular mass standards (in kDa) are indicated. B, purification of recombinant Rpm1-NBARC. A 0.5 μg portion of protein was applied and molecular mass standards (in kDa) are indicated. C, 1.3 μm PSiP-NBARC was assayed in 100 μl reactions in the presence of 100 μm ATP (upper panel), ADP (middle panel), or AMP (lower panel). Reactions were analyzed by HPLC. D, 1.3 μm PSiP-NBARC was assayed in 100 μl reactions in the presence of 100 μm GTP (upper panel), GDP (middle panel), or GMP (lower panel). Reactions were analyzed by HPLC. E, 10 nm Rpm1-NBARC was assayed in 100 μl reactions in the presence of 100 μm ATP (upper panel) or ADP (lower panel). Reactions were analyzed by HPLC.