Pf3-3R is inhibited to contact YidC.
E. coli MK6 cells expressing Pf3-24C (lane 1), Pf3-24C-3R (lane 2), Pf3-28C (lane 3), Pf3-28C-3R (lane 4), Pf3-33C (lane 5), or Pf3-33C-3R (lane 6) were coexpressed with YidC508C (lanes 1 and 2), YidC505C (lanes 3 and 4), or YidC476C (lanes 5 and 6), respectively. The cells were labeled with [35S]methionine for 30 s and immunoprecipitated with antiserum to Pf3 (A) or to YidC (B). The samples were analyzed by PAGE and phosphor imaging.