FIGURE 3.

Intermediate mechanosensitivity in triheteromeric NR1/NR2A/NR2B receptors. The cells were transfected with GFP-PSD95, with either NR1 and NR2B, or with NR1, NR2A and NR2B. Cells transfected with all subunits were either left untreated or treated with NR1/NR2B-specific antagonist, Ro 25-6981. A, normalized response to stretch was significantly decreased, but not eliminated, in cells expressing NR1, NR2A, and NR2B, demonstrating that these cells exhibit intermediate mechanosensitivity (*, p < 0.05 compared with NR1/NR2B). B, response to 100 μm NMDA application was not different. Treatment with Ro 25-6981 did not alter the response of NR1/NR2A/NR2B-transfected cells, suggesting that expressed NMDARs in these cells are primarily triheteromeric.