FIGURE 5.

NR2B C-terminal tail confers mechanosensitivity. The cells were transfected with NR2B or with truncation mutants of NR2B, NR2B-1036X, or NR1433X, which truncate the C-terminal tail distal to amino acid 1036 and 1433, respectively. A and B, normalized stretch response was significantly decreased in cells expressing NR2B-1036X (A), whereas response to 100 μm was not different (B). C and D, response to stretch was slightly increased in cells expressing NR2B-1433X (C), whereas response to NMDA stimulation was not different (D) among NR2B and NR2B-1433X. E and F, differential mechanosensitivity was also seen in cultures where PSD-95 was absent, where NR2B-1036X again demonstrated a reduced stretch response (E) and similar NMDA response (F), compared with wild type. This suggests that NR2B mechanosensitivity is conferred by the intracellular domain within amino acids 1036–1433 (*, p < 0.05 compared with NR1/NR2B response).