FIGURE 4.

DHT-induced histone modifications and transcription complex assembly on the TMPRSS2 gene. A, diagram of the regulatory region of the TMPRSS2 gene showing five putative AREs (circles) and their locations measured in bp relative to the TSS (arrow). ARE V is the main site of AR recruitment. B–E, after growth in hormone-free media for 3 days, C4-2B cells were treated with ethanol (EtOH) or 10 nm DHT for 4 h, and ChIP was performed with the indicated antibodies or no antibody (-ab) as a control, using primers for the indicated region of the TMPRSS2 gene. Each graph shows a single biological experiment that is representative of at least two independent biological experiments. Immunoprecipitated DNA was analyzed relative to input DNA (before immunoprecipitation), and values shown are mean ± S.D. of three technical qPCR replicates, using primers specific for the indicated region.