FIGURE 7.

Effect of p300 depletion on DHT-induced histone modifications and transcription complex assembly on the FKBP5 gene. A–B, C4-2B cells were transfected with siRNA against p300, and cells were treated with ethanol or DHT for 4 h, prior to ChIP analysis performed as in Fig. 4. Each graph shows a single biological experiment that is representative of at least two independent biological experiments. Immunoprecipitated DNA was analyzed relative to input DNA (before immunoprecipitation), and values shown are mean ± S.D. of three technical qPCR replicates, using primers specific for the indicated region.