FIGURE 2.
Effect of inhibition of ASIC-1 on cell cycle progression and whole-cell current of D54-MG glioma cells. A, flow cytometric analysis of D54-MG cells treated with PcTX-1, control peptide (Con_Pep), and benzamil (Benz) for 24 h. DNA content is shown as 2n and 4n in the x axis where 2n = cells in G0/G1 phase and 4n = cells in G2/M phase. The bar graph shows the percentage of cells in each cell cycle phase for the different experimental conditions, n ≥4. B, expression of p21Cip1 and p27Kip1 proteins in D54-MG cells treated with PcTX-1, control peptide, or benzamil for 24 h. β-Actin was used as a loading control. Accompanying bar graph shows the normalized density as compared with 2% FBS control n ≥6. IB, immunoblot. C, bar graph showing average amiloride-sensitive currents at −80 mV for D54-MG cells pretreated with PcTX-1, control peptide, or benzamil for 24 h. Currents were recorded in the continued presence of drug in the bath solution n ≥6. D, summary I/V curves showing basal current and that following addition of amiloride to the bath solution.