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Knocking down δENaC has no effect CKI expression. A, lysates from untransfected wild type D54-MG and D54MG-δENaC-DN cells were separated by SDS-PAGE and immunoblotting (IB). Each bar represents the normalized density of both bands compared with untransfected D54-MG cells, n ≥3. B, no difference in expression of p21Cip1 and p27kip1 proteins was detected between wild type D54-MG and D54MG-δENaC-DN cells. Wild type cells treated with benzamil were used as a positive control (n ≥6).
