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. 2011 Dec 14;287(6):4323–4334. doi: 10.1074/jbc.M111.295113

FIGURE 4.

FIGURE 4.

ARF6 activation upon CpG ODN stimulation. A, RAW-ARF6 cells were treated with either GpC ODN or CpG ODN for the indicated times. The cells were then lysed on ice and subjected to ARF6 pulldown assays with the GST-GGA1 fusion protein. The level of ARF6-HA-GTP was assessed by immunoblotting with anti-HA. B, RAW-ARF6 cells were treated with either GpC ODN or CpG ODN for 30 min. Cell lysates were incubated with 10 μg GST-GGA1 protein and then were subjected to an immunoprecipitation (IP )assay with anti-HA. The precipitated level of GST-GGA1 was determined by immunoblotting (WB) with anti-GST. C, RAW-ARF6 cells were transfected with control plasmids (psiRNA-lucGL3) or psiRNA-TLR9 plasmid for 24 h and then stimulated with GpC ODN or CpG ODN for 30 min. Cell lysates were subjected to ARF6 pulldown assays with the GST-GGA1 fusion protein. The level of ARF6-HA-GTP was assessed by immunoblotting with anti-HA. The experiment was repeated twice with similar results.