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. 2011 Dec 14;287(6):4323–4334. doi: 10.1074/jbc.M111.295113

FIGURE 7.

FIGURE 7.

ARF6 regulates cellular CpG ODN uptake. A, RAW264.7, RAW-ARF1T31N, or RAW-ARF6T27N cells were incubated with 0.5 μm CpG ODN-FITC for the indicated times. Cell fluorescence intensity of CpG ODN-FITC uptake was measured by flow cytometry. *, p < 0.01 for RAW-ARF6T27N versus RAW264.7. Data represent the mean ± S.D. of three experiments. B, RAW264.7, RAW-ARF1T31N, RAW-ARF6, RAW-ARF6T27N, or RAW-ARF6Q67L cells were incubated with 0.5 μm CpG ODN-FITC for 5 min. CpG ODN-FITC was detected by confocal microscopy. The experiment was repeated twice with similar results. Scale bar, 5 μm. C, RAW264.7 cells were pretreated with either CpG ODN or GpC ODN for the indicated times followed by 0.5 μm CpG ODN-FITC for 10 min. Cell fluorescence intensity of CpG ODN-FITC uptake was measured by flow cytometry. *, p < 0.01 for CpG ODN versus endotoxin-free Tris-EDTA buffer or GpC ODN.