FIGURE 3.

Akt Inhibitor IV suppresses HUCB cell-induced Prdx4 expression during OGD. qRT-PCR was performed to quantify mRNA expression of the antioxidant enzyme Prdx4. OLs were subjected to OGD and treated with HUCB cells in the presence or absence of Akt Inhibitor IV. HUCB cells increased Prdx4 gene transcript compared with OGD alone (n ≥ 7; *, p < 0.05). The addition of the Akt inhibitor with HUCB cells reduced the HUCB cell-induced elevation in Prdx4 mRNA (*, p < 0.05) back to levels detected in OLs treated with OGD or Akt inhibitor alone.