FIGURE 2.

H₂S enhances antigen-specific T cell activation. Murine CD4+ OT-II T cells (3 × 10⁶ cells) were activated with a combination of 1 μg/ml OVA2 peptide and 5 μg/ml anti-CD28 antibody in the presence of 300 nm Na₂S or vehicle in 1% O₂, and gene expression of IL-2 was examined by RT-PCR at 4, 10, and 24 h (A). Data are normalized to non-activated control for each treatment; n = 3, error bars indicate S.D. *, denotes p < 0.05. B, IL-2 receptor (CD25) was measured in the presence or absence of 100 nm Na₂S by flow cytometry. Data are representative of n = 2.