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. 2011 Dec 7;287(6):3850–3858. doi: 10.1074/jbc.M111.317404

FIGURE 5.

FIGURE 5.

Expression of PGAM1 mutant 3KQ (acetylated mimic) stimulates glycolytic flux. WT MYC-PGAM1, 3KQ, and 3KR were overexpressed in HEK293 cells. A, input blots (1% total lysate) were detected with anti-MYC antibody. B, glucose levels in HEK293 cells expressing PGAM1, PGAM1 3KQ, and PGAM1 3KR. C, lactate levels in HEK293 cells expressing PGAM1, PGAM1 3KQ, and PGAM1 3KR. D, alanine levels in HEK293 cells expressing PGAM1, PGAM1 3KQ, and PGAM1 3KR. Error bars represent S.E. (n = 3); *, p < 0.05.