FIGURE 6.

A, LPS-induced NF-κB activation of TLR4 mutant HEK293 cells were transiently co-transfected with a pMX-mMD-2, pMX-mCD14, and pMX-TLR4-IRES-GFP WT or mutant vector together with the pNFconluc reporter plasmid. After 24 h, the cells were stimulated overnight with 0, 10, 100, or 1000 ng/ml LPS. Luciferase measurements were performed in triplicate. B, effect of TLR4 mutant expression on IRF3/GAL4DBD activation. Data are presented as % of wild type TLR4 and present the median of three separate transfection experiments. All tested mutants affect the IRF3/DBD activation.