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. 2011 Dec 2;287(6):4088–4098. doi: 10.1074/jbc.M111.282350

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Competition between MAL and TRAM in MAPPIT. HEK293T cells were transiently co-transfected with plasmids encoding for the Mock or TLR4ic bait and the SVT (negative control), SH2β (positive control), and MAL (A) or TRAM prey (B). A, comparison of TLR4ic-MAL interaction with and without co-transfection of plasmid encoding for TRAM. Dashed arrow, TRAM overexpression specifically inhibits the MAPPIT signal for the TLR4ic-Mal interaction. B, comparison of the TLR4ic-TRAM interaction with and without co-transfection of plasmid encoding for MAL. Dashed arrow, MAL overexpression specifically inhibits the MAPPIT signal for the TLR4ic-TRAM interaction.