TABLE 4.
Titration microcalorimetry data for the interaction between BambL and fucosylated ligands
Experiments were performed twice, and ±S.D. values are expressed for KD and ΔH.
| Ligand | Fitting model | n | KD | −ΔG | −ΔH | TΔS |
|---|---|---|---|---|---|---|
| μm | kJ/mol | kJ/mol | kJ/mol | |||
| A-tri | 1 | 1.96 | 0.46 ± 0.02 | 36.0 | 53.1 ± 0.1 | −16.9 |
| H-type 5/FucLac | 1 | 2.03 | 0.76 ± 0.07 | 34.9 | 46.3 ± 0.2 | −11.3 |
| αMeFuc | 1 | 2.02 | 0.96 ± 0.03 | 34.3 | 47.8 ± 1.8 | −13.5 |
| H-di | 1 | 2.04 | 1.03 ± 0.08 | 34.2 | 45.7 ± 0.2 | −11.5 |
| XFG | 1 | 1.94 | 6.0 ± 0.5 | 29.8 | 53.0 ± 1.6 | −23.2 |
| H-type 2 | 1 | 1.96 | 7.5 ± 0.1 | 29.3 | 44.4 ± 0.7 | −15.2 |
| 2, S1 | 0.97 | 0.56 ± 0.01 | 35.7 | 47.8 ± 1.1 | −12.1 | |
| 2, S2 | 1.10 | 14.0 ± 0.3 | 27.7 | 39.7 ± 0.9 | −12.0 | |
| LeY | 1 | 2.00 | 11.1 ± 0.1 | 28.3 | 38.6 ± 0.4 | −10.3 |
| Lea | 1 | 2a | 18.2 ± 0.7 | 27.1 | 28.7 ± 0.1 | −1.6 |
| H-type1 | 1 | 2a | 26.1 ± 0.1 | 26.2 | 17.6 ± 0.1 | 8.6 |
| LeX | 1 | 1.97 | 34.8 ± 1.4 | 25.4 | 39.1 ± 0.2 | −13.6 |
| Leb | 1 | 2a | 63.7 ± 1.2 | 23.9 | 25.4 ± 0.3 | −1.5 |
| B-penta | 1 | 2a | 95.3 ± 1.8 | 23.0 | 25.9 ± 1.2 | −2.9 |
| A-penta | 1 | 2a | 120 ± 2 | 22.4 | 13.7 ± 0.1 | 8.7 |
a Stoichiometry value n was fixed during fitting procedure.