FIGURE 6.

SVCT-2 expression is modulated by the canonical nitric oxide pathway. Cultures were preincubated with (A) LY83583 (10 μm) or (B) KT5823 (500 nm) for 10 min. Then, cells were incubated with SNAP (100 μm) either in the presence or absence of the mentioned compounds for 80 min. Next, samples were prepared for Western blotting. D, cultures were incubated with 8-bromo-cGMP (1 mm) or Zaprinast (10 μm) for 80 min and also prepared for Western blotting. Quantification histograms are plotted in C and E in a SVCT-2/AKT ratio normalized to the control. Results in A, B, and D are representative images. Data in C and E are the mean ± S.E. of different and independent experiments; SNAP (n = 9), SNAP + LY83583 (n = 3), LY83583 (n = 3), SNAP + KT5823 (n = 3), KT5823 (n = 3), 8-bromo-cGMP (n = 3), Zaprinast (n = 3). ***, p < 0.001 (relative to control). ZAP, Zaprinast; CT, control.