TABLE 2.
Kinetic rates (kobs) per min of MvNei1 with double strand substrates (Tg:A, 5-OHU:G, or AP:A) determined under single-turnover conditions
The kinetic parameters were obtained from three independent experiments. Data obtained with 5-OHU:G and AP:A were fit with a double-exponential model, which yields two rate constants (k1 and k2). Standard deviations are shown. NA means not applicable.
| k1 | k2 | |
|---|---|---|
| WT | ||
| Tg:A | 0.541 ± 0.028 | NA |
| 5-OHU:G | 5.75 ± 2.36 | 0.107 ± 0.0316 |
| AP:A | 898.2 ± 179.2 | 27.3 ± 4.03 |
| E6A | ||
| Tg:A | 0.144 ± 0.006 | NA |
| 5-OHU:G | 6.76 ± 1.07 | 0.081 ± 0.0168 |
| AP:A | 523.1 ± 86.5 | 12.8 ± 2.98 |
| Y253F | ||
| Tg:A | 0.081 ± 0.004 | NA |
| 5-OHU:G | 6.98 ± 1.39 | 0.104 ± 0.0157 |
| AP:A | 1101.6 ± 371.6 | 62.7 ± 8.06 |