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. 2011 Dec 14;287(6):4299–4310. doi: 10.1074/jbc.M111.282152

FIGURE 5.

FIGURE 5.

MICA regulatory control site integrates input from both heat shock and NF-κB pathways. A, locations and sequences of the mutations introduced to the reporter constructs. Bases corresponding to the predicted binding sites for NF-κB or heat shock factor (HSE) sites are boxed and highlighted. Mutations designed to specifically disrupt either the NF-κB or HSE site in the corresponding reporter constructs are shown in boxed italics. B, different sets of mutations at the −130-bp NF-κB/HSE site selectively abolish the induction of the MICA promoter by NF-κB or heat shock. HeLa cells were transfected with the indicated reporter constructs bearing specific mutations to the −130-bp site together with either an NF-κB p65 expression vector (NF-κB) or empty control vector (Control and Heat Shock), and cells were harvested 48 h post-transfection for reporter assay (NF-κB and Control) or subjected to heat shock (Heat Shock) for 1 h at 42 °C followed by 5-h recovery at 37 °C before cells were harvested. The error bars represent standard deviations of three replicates. LUC, luciferase.