FIGURE 1.
BMPER expression in Trfhpx/hpx mice. A, qPCR shows relative Bmper and hepcidin mRNA levels (the results were normalized to the housekeeping gene RPL-19) in 10- and 11-week-old male Trfhpx/hpx mice (n = 3). Trfhpx/+ were used as control (C) mice (n = 3). The inset gel picture shows a semiquantitative RT-PCR assay for Bmper and hepcidin in the same samples. B, Western blots showing BMPER expression in the same mice as in A. C, BMPER staining (green) in liver sections from male, 7-week-old Trfhpx/hpx compared with age- and sex-matched control (Trfhpx/?), counterstain is propidium iodide (*, p < 0.003; †, p < 0.03, compared with control mice, Student's t test).