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. 2011 Dec 21;4:52. doi: 10.1186/1756-8722-4-52

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Copyright ©2011 Wudtiwai et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of 5-MF and 2'-MF on caspase-8 and -3 activities in MOLT-4 cells. Caspase-8 activity was measured in 5-MF- (A) and 2'-MF- (B) treated cells for various times in the presence or absence of TRAIL for 24 h. The IETD-AFC substrate was cleaved and produced fluorescence. Caspase-3 activity was measured in 5-MF- (C) and 2'-MF- (D) treated cells for various times and with or without TRAIL for 24 h. The DEVD-AFC substrate was used. N-fold increase of fluorescence intensity was compared to that of untreated (control) cells, which was calculated to be 1. *, p < 0.05, compared with control.