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. 2012 Feb 17;7(2):e30120. doi: 10.1371/journal.pone.0030120

Figure 9. Comparison of target gene expression in THP-1 cells and PBMCs treated with cigarette smoke extract (CSE).

Figure 9

We have previously shown that CSE increases the expression of HMOX1, TNF and IL8 in THP-1 cells and PBMCs. RNA was extracted from each sample and gene expression values measured using the Illumina HumanRef-8v3 BeadChip array. (A) Heat map representing normalized signal intensities of HMOX1, TNF and IL8 genes from THP-1 monocytes treated for 8 hours with RPMI-1640 (control 1–6) and 10% CSE (1–6). (B) Heat map representing normalized signal intensities of HMOX1, TNF and IL8 genes from PBMCs treated for 8 hours with RPMI-1640 (control 1–3), 3% and 10% CSE (1–3). (C) Heat map representing normalized signal intensities of HMOX1, TNF and IL8 genes from PBMCs treated for 24 hours with RPMI-1640 (control 1–3), 3% and 10% CSE (1–3). Heat maps were generated using GeneSpring GX11.0.2 software where, red denotes high expression and turquoise denotes low expression.