Figure 3. Curcumin treatment inhibits esophageal cancer cells spheroid formation.

(A) TE-7 and TE cells were grown in low adherent plates and treated with increasing concentrations of curcumin (0–50 µM) and performed for the spheroid assay. After one week, the spheroids were photographed. (B) Spheroid was counted and performed bar diagram. Curcumin treatment significantly inhibited esophageal cancer cells spheroids (*p<0.05). (C) The primary spheroids were collected and separated into single cells and replated. Curcumin treatment significantly inhibited esophageal cancer cells secondary spheroids (*p<0.05).