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. 2012 Feb 17;7(2):e30590. doi: 10.1371/journal.pone.0030590

Figure 4. Curcumin inhibits notch signaling and its downstream targets proteins.

Figure 4

(A) real-time reverse transcription-PCR analysis of total RNA from TE-7 cells following 30 µM of curcumin treatment for 24 h showed reduction in the expression of Notch-1, its ligand Jagged-1 and its target gene Hes-1 mRNA (*p<0.05). (B) lysates from curcumin treatment caused significant reduction in the expression of cleaved Notch-1, its ligand Jagged-1and its target gene Hes-1 protein levels in TE-7 cells. (C) TE-7 cells treated with 30 µM of curcumin for 24 h were subjected to immuno-fluorescent staining using anti-Notch-1, anti-Jagged-1 and anti-Hes-1 antibodies. Curcumin treatment resulted in lower levels of Notch-1 protein in the nucleus and reduced Jagged-1 and Hes-1 expression in TE-7 cells.