Phylogenetic and evolutionary analyses were performed using the neighbor-joining method by DNAMAN software. Additional sequences include Medicago truncatula (MtDFR1, AAR27014), Populus tremuloides (PtDFR, AY147903), Arabidopsis thaliana (AtDFR, AAA32783), Fragaria x ananassa (FaDFR, AAC25960), Malus x domestica (MdDFR, AAD26204), Vitis vinifera (VvDFR, CAA53578), Dianthus caryophyllus (DcDFR, CAA91924), Gerbera hybrid (GhDFR, CAA78930), Gentiana triflora (GtDFR, AA12736), Ipomoea nil (InDFR, BAA22072), Ipomoea purpurea (IpDFR, BAA74700), Nicotiana tabacum (NtDFR, ABN80437), Solanum lycopersicum (SiDFR, CAA79154), Petunia x hybrida (PhDFR, AAF60298), Vaccinium macrocarpon (VmDFR, AAL89714), Hordeum vulgare (HvDFR, AAB20555), Bromheadia finlaysoniana (BfDFR, AAB62873) and Ginkgo biloba (GbDFR, AAU95082).