Skip to main content
. 2012 Feb 17;7(2):e30540. doi: 10.1371/journal.pone.0030540

Figure 2. Characterization of rgBTV8 containing a marker mutation.

Figure 2

(A) SnaBI or XhoI digestion of Seg-2 RT-PCR products. SnaBI- or XhoI-digested RT-PCR products amplified from isolated RNA of strains rg- and wtBTV8 (passage 3) with primers btv8-S2f and btv8-S2r6 were separated on 1,5% agarose gel. Lanes 1–2 SnaBI-digestion: lane 1, wtBTV8; lane 2, rgBTV8. Lanes 3–4 XhoI -digestion: lane 3, wtBTV8; lane 4, rgBTV8. Marker sizes (in bp) are indicated on the right (TrackIt™ 100 bp DNA ladder Invitrogen). Sizes of SnaBI-digested fragments are indicated on the left (in bp). (B) Sequence electropherograms of the Seg-2 RT-PCR products of wt- and rgBTV8 including the Seg-2 SnaBI mutation in rgBTV8 at position 242 (nucleotide position in segment indicated above sequences).