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. 2012 Feb 17;7(2):e30540. doi: 10.1371/journal.pone.0030540

Figure 3. Characterization of rgBTV6 containing a marker mutation.

Figure 3

(A) NlaIV or LguI digestion of Seg-2 RT-PCR products. NlaIV or LguI-digested RT-PCR products amplified from isolated RNA of strains rg- and wtBTV6 (passage 3) with primers btv6-S2f8 and btv6-S2-2R were separated on 1,5% agarose gel. Lanes 1–2 NlaIV-digestion: lane 1, wtBTV6; lane 2, rgBTV6. Lanes 3–4 LguI-digestion: lane 3, wtBTV6; lane 4, rgBTV6. Marker sizes (in bp) are indicated on the left (TrackIt™ 100 bp DNA ladder Invitrogen). (B) Sequence electropherograms of the Seg-2 RT-PCR products of wt- and rgBTV6 including the Seg-2 LguI mutation in rgBTV6 at position 910 (nucleotide position in segment indicated above sequences).