Figure 4. Growth curve and plaque size of viruses rgBTV6, wtBTV6, rgBTV8 and wtBTV8.

(A) BSR monolayers were infected in duplicate by indicated viruses with 0.1 moi. At 24, 48 and 72 hours post infection, samples of 1 ml supernatant were taken. The virus titer in collected samples were determined by plaque-titration, expressed as pfu/ml, and plotted at a logarithmic scale. The bars represent the averages and standard errors from two replicates. (B) BSR monolayers were infected by indicated viruses in tenfold dilutions and grown for 2 days under overlay medium (EMEM complete with 1% methylcellulose). Plaques in appropriate comparable dilutions are displayed.