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. 2012 Feb 17;7(2):e30540. doi: 10.1371/journal.pone.0030540

Figure 5. Experimental infection of sheep with viruses rgBTV6, rgBTV8 and wtBTV8.

Figure 5

Four sheep per group were infected with 105 TCID50/ml rgBTV6 (i.m.) or 105 TCID50/ml rg- or wtBTV8 (s.c.). (A) body temperatures (mean values per group; lines) and clinical scores (per group; bars) were recorded daily. (B) the presence BTV genomic RNA was determined through a real-time Seg-10 RTPCR (mean values per group). (C) the presence of BTV specific antibodies in serum samples was determined with a BTV-VP7 blocking Elisa (ID-VET) and the mean blocking percentage per group was displayed as 100-value (sample).