Table 1. List of forward and reverse primers used for amplification of (parts of) genome segment 2 of BTV6 and BTV8.
primera | sequence | expected amplicon size (bp) |
BTV8S2f | GTTAAAATAGCGTCGCGATG | 964 |
BTV8S2r6 | GATGTTGGTACGCTCGTT | |
BTV6S2f8 | TGATCCACGACTCAATGGAC | 791 |
BTV-S2-2R | TTTCTGATGCGATCAATG |
All primers were supplied by Eurogentec b.v., Maastricht, Netherlands.