Table 2. Sequence differences between strain BTV8/2007 and BTV8/Neth2006.
| RNA segment | nucleotide positiona | BTV8Neth2006/04b | BTV8Neth2006c | BTV8Neth2007d | amino acid changee | Remarks |
| Seg-1 | 1128 | t | c | c | - | |
| 1226 | g | a | g | - | ||
| 1454 | g | a | a | - | ||
| 1491 | c | g | g | Q493E | ||
| 2051 | c | c | t | - | ||
| 2534 | c | c | t | - | ||
| 2634 | a | a | g | T875A | ||
| 3701 | c | c | t | - | ||
| 3939 | c | a | a | - | 3′UTR | |
| Seg-2 | 242 | a | a | g | - | |
| 443 | g | g | a | - | ||
| 470 | c | c | t | - | ||
| 1007 | c | c | a | - | ||
| 2084 | g | a | g | - | ||
| Seg-3 | 38 | g | a | a | - | |
| 62 | t | a | a | - | ||
| 113 | c | c | t | - | ||
| 1328 | t | t | c | - | ||
| 2471 | c | t | t | - | ||
| Seg-4 | 620 | g | g | a | - | |
| 1026 | a | a | t | M340L | ||
| 1324 | t | g | g | F439G | ||
| 1960 | a | g | g | - | 3′UTR | |
| 1969 | c | a | a | - | 3′UTR | |
| 1971 | t | g | g | - | 3′UTR | |
| Seg-5 | 1432 | c | t | c | - | |
| 1639 | c | c | t | - | ||
| 1768 | t | - | t | - | 3′UTR | |
| Seg-6 | 19 | c | t | t | - | 5′UTR |
| 144 | a | a | g | - | ||
| 546 | a | a | g | - | ||
| 1365 | c | c | t | - | ||
| 1383 | a | g | g | - | ||
| Seg-8 | 194 | g | a | a | D59N | |
| 781 | g | a | a | - | ||
| 1112 | g | - | g | 3′UTR | ||
| Seg-9 | 11 | - | ta | - | 5′UTR | |
| 22–24 | cta | gct | gct | L3A | ||
| 141 | g | g | a | A43T | ||
| 256–258 | gac | aga | aga | D81R | ||
| 413 | t | t | c | A133V | ||
| 863 | g | a | a | - | ||
| 1020–1022 | tct | ctc | ctc | - | 3′UTR | |
| 1024 | c | t | t | - | 3′UTR | |
| 1041–1044 | acat | taca | taca | - | 3′UTR |
Nucleotide position numbering from strain BTV8/Neth2007 (genbank accession numbers GQ506451–GQ506460).
Strain BTV8/Neth2006/04 [5] has been isolated on KC cells and passaged one time on BHK21 cells before analysis. The sequences from the genome segments correspond to genbank accession numbers AM498051–AM498060 for Seg-1 to Seg-10.
Strain BTV8/Neth2006 [25] is the same strain as BTV8/Neth2006/04 but has been passaged 2 times extra on BHK21 cells. The sequences from the genome segments correspond to genbank accession numbers FJ183374–FJ183383 for Seg-1 to Seg-10.
BTV8/net07/e1/bhk3 (described in this paper) was isolated on eggs and was further passaged 3 times on BHK21 cells before analysis. This sequence matches completely to sequences determined by Maan et al (2010). See also footnote a.
Amino acid position numbering from strain BTV8/Neth2007.