Figure 2. Assessment of participation of the nitrergic system on sperm release induced by anandamide.

Sperm cells and BOEC were co-cultured and then incubated for 15 min with BSA-free sp-TALP alone (control), AEA (1 nM) or MetAEA (1.4 nM) and L-NAME (1 µM; NO synthase inhibitor) or Hemoglobin (Hb) (30 µg/ml; NO scavenger). Bars indicate the number of spermatozoa that remained attached to the monolayers and represent the mean ± S.E.M. of bound spermatozoa/0.11 mm² monolayer (n = 6), a≠b p<0.05.