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. 2012 Feb 17;7(2):e30671. doi: 10.1371/journal.pone.0030671

Figure 3. Effect of L-NAME or Hemoglobin on bull sperm capacitation induced by AEA.

Figure 3

Spermatozoa were incubated for 45 min at 38.5°C in 0.3% BSA sp-TALP medium with AEA (1 nM) and L-NAME (1 µM) or Hb (30 µg/ml). Bars indicate the percentage of capacitated spermatozoa (A: % pattern B of CTC; B: % acrosome reacted spermatozoa). A: Assessment of sperm capacitation by CTC; T0 and T45: 0.3% BSA sp-TALP at 0 and 45 min (control) incubation respectively (n = 6). B: Assessment of sperm capacitation by LPC-induced acrosome reaction (AR)-PSA-FITC. T45: 0.3% BSA sp-TALP (control). After capacitation, spermatozoa were incubated for 15 min either with or without LPC to induce AR. Bars show percentage of spermatozoa that underwent LPC-induced AR minus the percentage of spermatozoa that underwent spontaneous AR (n = 6). Data are expressed as mean ± SEM. a≠b, p<0.05.