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. 2012 Feb 17;7(2):e30671. doi: 10.1371/journal.pone.0030671

Figure 5. Determination of NO levels in bull spermatozoa.

Figure 5

Sperm samples were incubated for 60 min at 38.5°C in 0.3% BSA sp-TALP containing 0.1 mM L-Arginine and 5 µM of DAF-FM diacetate and untreated (control) or treated with MetAEA (1.4 nM), MetAEA+L-NAME (1 µM). Spermatozoa were fixed and the fluorescent complex was measured by flow cytometry. A) A representative photograph showing fluorescence in spermatozoa, indicating the content of intracellular NO (Magnification, ×600); B) Fluorescence data are expressed as mean fluorescence (percentage of control at 45 min incubation, control adjusted to 100%). Data are expressed as mean ± SEM (n = 8). a≠b, p<0.05.