Figure 6. Participation of CB1 and TRPV1 in NO production during bull sperm capacitation by AEA.

Sperm samples were incubated for 60 min at 38.5°C in 0.3% BSA sp-TALP containing 0.1 mM L-Arginine and 5 µM of DAF-FM diacetate and untreated (control) or treated with MetAEA (1.4 nM) and/or SR141716A (SR1: CB1 antagonist (0.1 nM)) or Capsazepine (CZP: TRPV1 antagonist (10 nM)). Spermatozoa were fixed and the fluorescent complex was measured by flow cytometry. Fluorescence data are expressed as mean fluorescence (percentage of control at 45 min incubation, control adjusted to 100%). Data are expressed as mean ± SEM (n = 5). a≠b, p<0.05.