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Spermatozoa were incubated with the primary antibodies against nNOS (panel A) or eNOS (panel C); B and D) phase contrast. Controls were performed incubating sperm cells with IgG fractions from non-immunized rabbits at the same concentration that the primary antibody (data not shown) (n = 3); Scale bar: 10 µm (Magnification, ×600). Arrows indicate the immunoreactive staining of the NOS antibodies.
